House Bill 282 was defeated Wednesday by a 33-24 vote in the Wyoming House of Representatives.
The bill would have added a 50 cent per pack increase to cigarettes sold in Wyoming.
Currently, Wyoming levies a tax of 60 cents per pack, the bill would have raised it to a $1.10 tax.
A separate bill that would have increased taxes by $1.00 per pack failed earlier in the House Revenue Committee.
The bill would have subjected electronic cigarettes to state taxes as well.